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This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.，推荐阅读搜狗输入法2026获取更多信息
Hard difficulty hints, answers for Feb. 26 PipsNumber (10): Everything in this space must add up to 10. The answer is 4-2, placed vertically; 6-0, placed horizontally.。旺商聊官方下载是该领域的重要参考